Biolegend, 148403, PE anti-mouse/human SEMA4A Antibody, 25μg

Semaphorin 4A (SEMA4A) is a class IV semaphorin of soluble and transmembrane proteins originally identified as SemB. It is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain, and a Sema domain. SEMA4A is expressed on dendritic cells, activated B cells, and T cells. It is also found on different tissues, such as those in brain, lung, kindney, spleen, and testis. In the immune system, SEMA4A functions as an activator in the T-cell-mediated immune response and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo . Like other semaphorin family members it is also involved in guidance of axonal migration during neuronal development and in immune responses.
25μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse SEMA4A-Fc proteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for relevant formats) include: ELISA1.
Application References(PubMed link indicates BioLegend citation): Kumanogoh A. et al. 2005. Immunity. 22:305. (ELISA)
Product Citations: Niven J, et al. 2019. Cell Rep. 28:21. PubMed
RRID: AB_2565286 (BioLegend Cat. No. 148403) AB_2565287 (BioLegend Cat. No. 148404)
Structure: Single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a SEMA domain.
Distribution: Dendritic cells, T cells, B cells, brain, lung, kindney, spleen and testis
Function: An activator in T-cell-mediated immune response. Suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. It is also involved in guidance of axonal migration during neuronal development.
Cell Type: Dendritic cells, T cells, B cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules
Antigen References: 1. Kumanogoh A, et al. 2002. Nature. 419:629. 2. Kumanogoh A, et al. 2005. Immunity. 22:305. 3. Toyofuku T, et al. 2007. EMBO J. 26:1373. 4. Delgoffe GM, et al. 2013. Nature. 501:252.
Gene ID: 2035164218
UniProt: View information about SEMA4A on UniProt.org
Clone: 5E3/SEMA4A
Regulatory Status: RUO
Other Names: Semaphorin 4A, SEMAB, Sema B, Semaphorin B, RP35, CORD10, SEMA 4A
Isotype: Mouse IgG1, λ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.