Biolegend, 148902, Purified anti-mouse/rat CD161f (NKPR1f) Antibody, 100μg

CD161, also known as NKRP1f, is mainly expressed on NK cells with little to no expression on T or B cells. NKRP1f on the surface of dendritic cells can bind to Clr-g expressed on activated T cells and result in production of IL-2 and proliferation of T cells. NKRP1f may also play a role in the formation of synapses between T cells and antigen presenting dendritic cells.
100μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: NKRP1f transfected YB2/0 rat plasmacytoma cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Mortazavi F, et al. 2019. Bio Protoc. e3321:9. PubMed
RRID: AB_2564270 (BioLegend Cat. No. 148902)
Structure: CLR family, 24.7 kD, ITIM-less activation receptor
Distribution: NK cells, dendritic cells
Function: Facilitates IL-2 production and proliferation of T cells and may contribute to the formation of immunological synapses.
Ligand/Receptor: Clr-g
Cell Type: NK cells, Dendritic cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: CD Molecules
Antigen References: 1. Skálová T, et al. 2012. J. Immunol. 189:4881.2. Tian W, et al. 2005. Cell Immunol. 234:39. 3. Plougastel B, et al. 2001. Immunogenetics. 53:592. 4. Kveberg L, et al. 2011. Immunogenetics. 63:429.
Gene ID: 232408
UniProt: View information about CD161f on UniProt.org
Clone: C8
Regulatory Status: RUO
Other Names: Killer cell lectin-like receptor subfamily B member 1F (Klrb1f), Nkr-p1f, Nkrp1-f, A630024B12Rik
Isotype: Rat IgG1, κ