Biolegend, 149523, Alexa Fluor® 488 anti-mouse CD16.2 (FcγRIV) Antibody, 25μg

FcγRIV, also known as CD16.2, is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16.2 is the mouse homolog of human FcγRIIIA. CD16.2 is a low-affinity IgE receptor for all allotypes and the ligation of FcγRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and is involved in lung inflammation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: FCγR4 —EC domain fusion with IgG1 Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).
Application References(PubMed link indicates BioLegend citation): Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block) Nimmerjahn F, et al. 2005. Immunity 23:41.
Product Citations: Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
RRID: AB_2687087 (BioLegend Cat. No. 149523) AB_2687088 (BioLegend Cat. No. 149524)
Structure: 22.4 kD
Distribution: Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine
Function: Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory.
Interaction: FCγR4 –EC domain fusion with IgG1 Fc
Ligand/Receptor: IgG2a and IgG2b
Cell Type: B cells, Leukocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Mechetina LV, et al. 2002. Immunogenetics. 54:463-8. Nimmerjahn F, et al. 2005. Immunity. 23:41-51. Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34.
Gene ID: 246256
UniProt: View information about CD16.2 on UniProt.org
Clone: 9E9
Regulatory Status: RUO
Other Names: FcgRIV, Fcgr4, Fcrl3, CD16-2, FcgRIV, Fcgr3a, FcgammaRIV, 4833442P21Rik, Fc gamma RIV
Isotype: Armenian Hamster IgG