Biolegend, 149702, Purified anti-mouse Granzyme A Antibody, 100μg

Granzyme A is a serine protease that is similar to Granzyme B. However, unlike granzyme B, granzyme A is a homodimer. It is expressed in cytotoxic T cells and natural killer cells (NK), but is not essential for induction of apoptosis or DNA fragmentation. Cell death induced by granzyme A is slower and morphologically different than the cell death induced by granzyme B. It plays a crucial role in infection-mediated inflammation as well as immunological responses against viruses, bacteria, and tumor cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length recombinant granzyme A of mouse origin.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Fehniger TA, et al. 2007. Immunity 26:798. (ICFC)
Product Citations: Anthony SM, et al. 2021. eLife. 10:00. PubMed Yang M, et al. 2020. Oncoimmunology. 9:1708064. PubMed
RRID: AB_2565308 (BioLegend Cat. No. 149702)
Structure: 29 kD, Homodimer, Serine protease
Distribution: Cytotoxic T cells and natural killer cells (NK); low on granulocytes, B cells, and activated dendritic cells (DCs).
Function: Induction of cell death and apoptosis.
Cell Type: T cells, NK cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Proteases, Enzymes and Regulators
Antigen References: 1. Susanto O, et al. 2013. Cell Death Differ. 20:1183. 2. Ebnet K, et al. 1995. EMBO J. 14:4230. 3. Jenne DE, et al. 1988. Immunol. Rev. 103:53.
Gene ID: 14938
UniProt: View information about Granzyme A on UniProt.org
Clone: 3G8.5
Regulatory Status: RUO
Other Names: Hf, SE1, TSP1, Ctla3, TSP-1, Ctla-3, AW494114
Isotype: Mouse IgG2b, κ