Biolegend, 150004, Alexa Fluor® 647 anti-mouse Blimp-1 Antibody, 100μg

Blimp-1, also known as PRDM1, is a 98 kD protein containing 5 Kruppel-type zinc finger domains. Blimp-1 represses the transcription factors BCL6 and c-Myc. It is the master regulator of terminal B cell differentiation and is also involved in the differentiation and homeostasis of T cells and natural killer (NK) cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Amino acids 255-395 from mouse Blimp-1 fused with GST.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality testedWB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Product Citations: Chen HY, et al. 2022. Elife. 11:. PubMed Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed Snell LM, et al. 2018. Immunity. 49:678. PubMed Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed Roy K, et al. 2019. Immunity. 50:616. PubMed Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed Cho S, et al. 2018. Nat Commun. 9:2757. PubMed Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
RRID: AB_2565617 (BioLegend Cat. No. 150003) AB_2565618 (BioLegend Cat. No. 150004)
Structure: Five Kruppel-type zinc finger domains, 98 kD.
Distribution: Plasma cells, CD4+ and CD8+ effector/memory T cells, and natural killer cells.
Function: Terminal differentiation of B cells, effector/memory T cells, role in natural killer cells (NK) and T cell homeostasis, and repressor of BCL6 and c-Myc.
Cell Type: Plasma cells, T cells, NK cells, B cells
Biology Area: Cell Biology, Immunology, Transcription Factors
Antigen References: 1. Nakaki F, et al. 2013. Nature 501:222. 2. Crotty S, et al. 2010. Nat. Immunol. 11:114. 3. Zhao WL, et al. 2008. Blood 111:3867. 4. Climmino L, et al. 2008. J. Immunol. 181:2338. 5. Martins, G and Clarne, K. 2008. Annu. Rev. Immunol. 26:133.
Gene ID: 12142
UniProt: View information about Blimp-1 on UniProt.org
Clone: 5E7
Regulatory Status: RUO
Other Names: PR domain zinc finger protein 1, B lymphocyte-induced maturation protein-1, PRDM1, BLIMP1, PRDI-BF1, ZNFPR1A1, PRDM-1, BLIMP1
Isotype: Rat IgG2a, κ