Biolegend, 150429, KIRAVIA Blue 520™ anti-mouse CD20 Antibody, 25μg

CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca 2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD20 - transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm. KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.Sony and the Sony logo are registered trademarks of Sony Corporation.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_2892306 (BioLegend Cat. No. 150429) AB_2892306 (BioLegend Cat. No. 150430)
Structure: Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
Distribution: B cells and a subset of T cells.
Function: Regulates B-cell activation and proliferation.
Interaction: Associated with MHC class I and II, CD53, CD81, and CD82.
Cell Type: B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Morsy DE, et al. 2013. J. Immunol. 191:3112. 2. Lund FE, Randall TD. 2010. Nat. Rev. Immunol. 10:236. 3. Beers SA, et al. 2010. Blood 115:5191. 4. Kuijpers TW, et al. 2010. J. Clin. Invest. 120:214.
Gene ID: 12482
UniProt: View information about CD20 on UniProt.org
Clone: SA275A11
Regulatory Status: RUO
Other Names: Ms4a1, Ly-44
Isotype: Rat IgG2b, κ