Biolegend, 151806, APC anti-mouse TSLPR (TSLP-R) Antibody, 100μg

TSLPR, also known as the thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a 40kD type I membrane receptor that forms a functional heterodimeric complex with IL-7R and binds with TSLP. TSLPR is required for proper protein folding and a box1 motif important for association with the JAKs. TSLPR is expressed in myeloid cells, dendritic cells, B cells and activated monocytes. It is weakly expressed in T cells. Expression has also been reported in heart, skeletal, muscle, and kidney tissues. Ligand receptor interactions have been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in allergy diseases.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: TSLP Receptor Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Isaksen DE, et al. 2002. J. Immunol. 168:3288. (FC) Bell BD, et al. 2013. Nat. Immunol. 14:364. (FC) Iseki M, et al. 2012. International Immunology 24:183. (FC) Astrakhan A, et al. 2007. Nature Immunology 8:522. (FC)
RRID: AB_2650788 (BioLegend Cat. No. 151805) AB_2650789 (BioLegend Cat. No. 151806)
Structure: Type I membrane receptor, forms functional heterodimeric complex with IL-7R to bind TSLP. Contains WSXWS motif required for proper protein folding and a box1 motif that is important for association with the JAKs.
Distribution: Expressed preferentially in myeloid cells including dendritic cells, B cells, activated monocytes, weakly expressed in T cells. Also expressed in heart, skeletal, muscle, and kidney cells.
Function: Binds to TSLP to stimulate cell proliferation.
Ligand/Receptor: TSLP
Cell Type: Dendritic cells, B cells, Monocytes
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: 1. Reche PA, et al. 2001. J. Immunol. 167:336. 2. Tonozuka Y, et al. 2001. Cytogenet. Cell Genet. 93:23. 3. Zhang W, et al. 2001. Biochem. Biophys. Res. Commun. 281:878. 4. Wang YH, et al. 2006. Immunity 24:827.
Gene ID: 57914
UniProt: View information about TSLPR on UniProt.org
Clone: 22H9
Regulatory Status: RUO
Other Names: Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R
Isotype: Rat IgG2a, κ