Biolegend, 152002, Purified anti-mouse CD319 Antibody, 100μg

CD319/CD319/CS1/CRACC/SLAMF7 is a 66 kD transmembrane protein belonging to the CD2 subset of the immunoglobulin superfamily; it is expressed on most NK cells, B cells, and subsets of T cells. CD319 activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT) and inhibits immune responses in the absence of SAP-family adaptors or when bound to SH-domain molecules.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CD319-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested Direct ELISA, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2616915 (BioLegend Cat. No. 152002)
Structure: Extracellular domain with one Ig like V-set domain and one Ig like C2-set domain, and an cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs.
Distribution: Spleen, lymph node, bone marrow, testis, thymus; NK cells, B cells, and subsets of T cells.
Function: Activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT).
Interaction: Interacts (via ITSM phosphorylated on Tyr-302) with SH2D1B, PTPN6/SHP-1, PTPN11/SHP-2, INPP5D/SHIP1, CSK and FYN, and SAP-family adaptors (SAP, EAT-2, ERT).
Cell Type: B cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Li Y, et al. 2013. PLoS One 8(9):e76681. 2. Dongre P, et al. 2013. Mol. Immunol. 54:254-63. 3. Tovar V, et al. 2002. Immunogenetics 54:394. 4. Dong Z, et al. 2009. Nat. Immunol. 10:973.
Gene ID: 75345
UniProt: View information about CD319 on UniProt.org
Clone: 4G2
Regulatory Status: RUO
Other Names: 19A, CS1, CD319, CRACC, SLAMF7
Isotype: Rat IgG1, κ