Biolegend, 153010, KIRAVIA Blue 520™ anti-mouse/human CD282 (TLR2) Recombinant Antibody, 100μg

CD282 (Toll-like receptor 2, TLR2) is an 85kDa, type I transmembrane protein, with 14 leucine-rich repeats (LRR) in the extracellular domain and a toll/IL-1R (TIR) in the cytoplasmic region. TLR2 is expressed by monocytes/macrophages, dendritic cells, granulocytes, endothelial cell and epithelial cells. TLR2 associates with TLR1 and TLR6 in the cell surface and is involved in the recognition of pathogen-associated molecular patterns (PAMPs) such as peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, and LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.
100μg
Verified Reactivity: Mouse, Human
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Recombinant extracellular domain of murine TLR2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm. KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.Sony and the Sony logo are registered trademarks of Sony Corporation.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Blocking of IL-6 and TNF-a production induced by P3CSK4 on RAW264.7 cells was observed during development.
RRID: AB_2894420 (BioLegend Cat. No. 153009) AB_2894420 (BioLegend Cat. No. 153010)
Structure: Type I transmembrane protein, 14 leucine-rich repeats (LRR) in the extracellular domain, the intracellular domain contains a toll/IL-1R (TIR) domain.
Distribution: Monocytes/macrophages, dendritic cells, granulocytes, endothelial cell, epithelial cells.
Function: Activates innate host defense, induces production of pro-inflammatory cytokines.
Interaction: TLR1, TLR6 , MYD88 and TRAF6.
Ligand/Receptor: Peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, as well as different LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.
Cell Type: Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Biswas S, et al. 2016. Blood. 127:2618. 2. Wang Y, et al. 2016. Proc. Natl. Acad. Sci. USA 113:884. 3. Ramstead AG, et al. 2016. Infect. Immun. 84:940. 4. Scheeren FA, et al. 2014. Nat. Cell. Biol. 16:1238. 5. Barbalat R, et al. 2009. Nat. Immunol. 10:1200.
Gene ID: 240887097
UniProt: View information about CD282 on UniProt.org
Clone: QA16A01
Regulatory Status: RUO
Other Names: Toll-like receptor 2, TLR-2, TIL4, CD282, TLR2
Isotype: Mouse IgG1, κ