Biolegend, 153305, APC anti-mouse Folate Receptor β (FR-β) Antibody, 25μg

Folate receptor beta (FR-β) is a member of a family of folate binding receptors that have diverse structural identities but mediate transport of folates into cells. FR-β is a GPI-anchored protein with high affinity for folic acid. It is postulated that this receptor function as folate scavengers when folate supply is low or rapid cell growth requires elevated uptake of folate for methylation reactions including DNA biosynthesis. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Folate receptor β transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Nagai T, et al. 2009. Cancer Immunol Immunother. 1577-86.
Product Citations: Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed Zhang F, et al. 2020. EMBO Mol Med. e12034:12. PubMed Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed Roy AG, et al. 2021. Int J Mol Sci. 22:. PubMed Wallace-Povirk A, et al. 2022. Sci Rep. 12:11346. PubMed
RRID: AB_2721312 (BioLegend Cat. No. 153305) AB_2721313 (BioLegend Cat. No. 153306)
Structure: 30 kD, seven disulfide bonds
Distribution: Placenta, monocytes, tissue resident macrophages
Function: Transport of folate into cells
Ligand/Receptor: Folic Acid
Cell Type: Macrophages, Monocytes
Antigen References: 1. Elwood PC, 1989, J Biol Chem, 264:14893. 2. Shen F, et al, 1994, Biochemistry, 8;33:1209. 3. Yi YS, 2016, Immune Netw,16:337.
Gene ID: 14276
UniProt: View information about Folate Receptor beta on UniProt.org
Clone: 10/FR2
Regulatory Status: RUO
Other Names: FR-β
Isotype: Rat IgG2a, κ