Biolegend, 154202, Purified anti-mouse CD25 Antibody, 500μg

CD25, also known as IL-2 low affinity receptor is a type I, 55 kDa, transmembrane glycoprotein. CD25 It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: HT-2 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedBlock, Depletion, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Inhibition of IL-2 dependent proliferation of HT2 cells, immunoprecipitation of CD25 from cell lysates, in vivo depletion of CD25+ cells.
Application References(PubMed link indicates BioLegend citation): Malek TR, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:5694. (FC, Block, IP) Couper KN. 2007. J. Immunol. 178:4136. (Depletion)
Product Citations: Jayachandran R, et al. 2019. Immunity. 50:152. PubMed Zhou D, et al. 2022. Nat Commun. 13:5174. PubMed
RRID: AB_2716173 (BioLegend Cat. No. 154202)
Structure: Type I, 55 kD, transmembrane glycoprotein
Distribution: Activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells
Ligand/Receptor: IL-2
Cell Type: B cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Huss DJ, et al. 2015. J. Immunol. 194:84. 2. Hartmann FJ, et al. 2014. Nat. Commun. 5:5056. 3. Codarri L, et al. 2007. J. Exp. Med. 204:1533. 4. Read S, et al. 2000. J. Exp. Med. 192:295. 5. Taniguchi T, et al. 1993. Cell 73:5.
Gene ID: 3559
UniProt: View information about CD25 on UniProt.org
Clone: 7D4/CD25
Regulatory Status: RUO
Other Names: IL-2Rα, IL2RA, IL-2Ra, Ly-43, p55, Tac
Isotype: Rat IgM, κ