Biolegend, 154406, PE anti-mouse Perforin Antibody, 100μg

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse perforin transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S16009B reacts with perforin from both C57BL/6 and BALB/c strains.
Product Citations: Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed Zhang B, et al. 2021. Nature. 599:471. PubMed Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed
RRID: AB_2721640 (BioLegend Cat. No. 154405) AB_2721641 (BioLegend Cat. No. 154406)
Structure: 70 kD Protein that oligomerizes for pore formation.
Distribution: CTL, NK (Cytoplasmic Granules)
Function: Target cell lysis
Cell Type: T cells, NK cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: Podack ER, et al, 1988, Immunol Rev, 103:203 Konjar S, et al, 2010. Immunology, 131:257 Trapani JA, et al, 2002, Nat Rev Immunol, 2:735 Osinska I, et al. 2014, Cent Eur J Immnol, 39:109
Gene ID: 18646
UniProt: View information about Perforin on UniProt.org
Clone: S16009B
Regulatory Status: RUO
Other Names: Prf1, Pfp, Pfn, Pfn-1
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.