Biolegend, 155507, APC anti-mouse CD170 (Siglec-F) Antibody, 25μg

CD170, also known as Siglec-F and Siglec-5, is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family of type I single pass transmembrane proteins.  Siglec-F has four extracellular Ig-like domains and two cytoplasmic ITIM motifs and preferentially binds [alpha]-2,3-linked sialic acid. Siglec-F is expressed on eosinophils, alveolar macrophages and intestinal microfold (M) cells and induces apoptosis of lung eosinophils during allergic asthma.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Product Citations: Larson-Casey JL, et al. 2023. JCI Insight. 8:. PubMed Gonçalves R, et al. 2023. iScience. 26:105972. PubMed He J, et al. 2022. Front Immunol. 12:760138. PubMed Larson-Casey JL, et al. 2021. FASEB J. 35:e21675. PubMed Pionnier NP, et al. 2019. Nat Commun. 10:1429. PubMed Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed Daneshmandi S, et al. 2021. Elife. 10:. PubMed Larson-Casey JL, et al. 2021. J Biol Chem. :100810. PubMed Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed Larson–Casey JL, et al. 2019. J Clin Invest. 129:4962. PubMed Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
RRID: AB_2750236 (BioLegend Cat. No. 155507) AB_2750237 (BioLegend Cat. No. 155508)
Structure: Member of the Sialic acid-binding Ig-like lectin family, type I, single pass, transmembrane protein, 4 extracellular Ig-like domains, 2 ITIM motifs in the cytoplasmic domain
Distribution: Eosinophils, alveolar macrophages, intestinal microfold (M) cells
Function: Induces apoptosis in eosinophils
Ligand/Receptor: [alpha]-2,3-linked sialic acid
Cell Type: Eosinophils, Macrophages
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: Gicheva N, et al. 2016. Biochem. Biophys. Res. Commun. 479:1. Kiwamoto T, et al. 2015. J. Allergy Clin. Immunol. 135:1329. Suzukawa M, et al. 2013. J. Immunol. 190:5939. Patnode ML, et al. 2013. J. Biol. Chem. 288:26533.
Gene ID: 233186
UniProt: View information about CD170 on UniProt.org
Clone: S17007L
Regulatory Status: RUO
Other Names: Siglec-5
Isotype: Rat IgG2a, κ