Biolegend, 156102, Purified anti-mouse TIGIT (Vstm3) Antibody, 500μg

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and transmembrane domain-containing protein 3 (Vstm3), is a 26 kD, type I transmembrane protein and member of the CD28 family. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. Its binding partners include CD155 (PVR) and CD112 (PVRL2). TIGIT is a negative regulator of NK and T cell activation. Engagement of TIGIT by dendritic cells results in their differentiation into a tolerogenic phenotype, with an increased secretion of IL-10 and a diminished production of IL-12. Mice deficient for TIGIT are more susceptible to autoimmune disease.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Hamster
Immunogen: Mouse TIGIT Tetramers
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: It was reported that this clone can block the interaction between mouse TIGIT and CD155.
Application References(PubMed link indicates BioLegend citation): Joller N, et al. 2011. J. Immunol. 186:1338.
Product Citations: Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed Hezaveh K, et al. 2022. Immunity. 55:324. PubMed
RRID: AB_2750250 (BioLegend Cat. No. 156102)
Structure: Type I transmembrane protein, 26 kD, member of the CD28 family. Contains 1 Ig-like domain and 1 cytoplasmic ITIM motif.
Distribution: Activated T cells, follicular T helper cells, NK, memory T cells, Tregs.
Function: Negative regulator of T cell activation and proliferation. Induction of tolerogenic dendritic cells.
Ligand/Receptor: CD155 (PVR) and CD112 (PVRL2).
Cell Type: Dendritic cells, NK cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: Adhesion Molecules, Immune Checkpoint Receptors
Antigen References: Levin SD, et al. 2011. Eur. J. Immunol. 41:902. Yu X, et al. 2009. Nat. Immunol. 10:48. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. USA 106:17858
Gene ID: 100043314
UniProt: View information about TIGIT on UniProt.org
Clone: 4D4/mTIGIT
Regulatory Status: RUO
Other Names: TIGIT, Vstm3, WUCAM, VSIG9
Isotype: Hamster IgG