Biolegend, 158211, KIRAVIA Blue 520™ anti-mouse CD127 (IL-7Rα) Antibody, 25μg

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse IL7R-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm. KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.Sony and the Sony logo are registered trademarks of Sony Corporation.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone S18006K is able to completely block the binding of clone SB/199 and partially block the binding of clone A7R34 to IL-7Rα.
RRID: AB_2922490 (BioLegend Cat. No. 158211) AB_2922490 (BioLegend Cat. No. 158212)
Structure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes
Biology Area: Immunology, Signal Transduction
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Sudo T, et al. 1993. Proc Natl Acad Sci USA. 90:9125. Okuno Y, et al. 2002. Proc Natl Acad Sci USA. 99:6246. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403.
Gene ID: 16197
UniProt: View information about CD127 on UniProt.org
Clone: S18006K
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Rat IgG2b, λ