Biolegend, 160703, PE anti-mouse IL-31 Antibody, 25μg

IL-31, mainly produced by activated CD4+ T cells, is a helical cytokine belonging to the gp130/IL-6 cytokine family. IL-31 is closely related in structure to IL-6, IL-11, and IL-27. Binding of IL-31 to its receptor activates JAK/STAT, PI3K/AKT, and MAPK pathways. IL-31 acts on a broad range of immune and non-immune cells and thus possesses potential pleiotropic physiological functions, such as regulating hematopoiesis and immune response. Transgenic mice overexpressing IL-31 developed severe dermatitis, indicating that IL-31 is involved in skin immunity.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse IL-31
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2876573 (BioLegend Cat. No. 160703) AB_2876573 (BioLegend Cat. No. 160704)
Structure: Member of IL-6 family of cytokines
Distribution: Subset of CD4 T cells
Ligand/Receptor: IL-31Ra/OSMR
Cell Type: Lymphocytes, T cells, Th2, Thymocytes
Biology Area: Adaptive Immunity, Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: McCandless EE, et al. 2014. Vet. Immunol. Immunopathol. 157:42. Arai I, et al. 2013. Exp. Dermatol. 22:669. Dai X, et al. 2013. PLoS One. 8:e67666. Stott B, et al. 2013. J. Allergy Clin. Immunol. 132:446. Hartmann K, et al. 2013. J. Allergy Clin. Immunol. 132:232. Baron JM and Lüscher B. 2012. Acta. Derm. Venereol. 92:5
Gene ID: 76399
UniProt: View information about IL-31 on UniProt.org
Clone: W17037B
Regulatory Status: RUO
Other Names: Interleukin-31
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.