Biolegend, 165402, Purified anti-mouse CD366 (Tim-3) Recombinant Antibody, 100μg

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b + macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
100μg
Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone QA20A20 completely blocks the binding of reference clone RMT3-23 on target cells.
RRID: AB_2924497 (BioLegend Cat. No. 165402)
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain with a predicted molecular weight of 31 kD; can exist as a soluble form lacking mucin and transmembrane domains.
Distribution: Expressed on activated Th1 lymphocytes and CD11b+ macrophages
Function: May play a role in the development of immune responses and the development of Th1-mediated responses
Interaction: Macrophages, Th1
Ligand/Receptor: Putative ligand on resting CD4+ lymphocytes
Cell Type: Dendritic cells, Macrophages, Th1
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: Sabatos CA, et al. 2003. Nat Immunol. 4:1102-10. Kuchroo VK, et al. 2003. Nat Rev Immunol. 3:454-62. Mooney L, et al. 2002. Nature. 415:536-41.
Gene ID: 171285
UniProt: View information about CD366 on UniProt.org
Clone: QA20A20
Regulatory Status: RUO
Other Names: CD366, Tim-3, Hepatitis A virus cellular receptor 2 homolog (HAVcr-2)
Isotype: Mouse IgG1, κ