Biolegend, 166703, Ultra-LEAF™ Purified anti-mouse CD90 (Thy1) Antibody, 100μg

CD90, also known as Thy-1, is a 25-35 kD immunoglobulin superfamily member. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells, but not on B cells. CD90 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90 is involved in costimulation of lymphocyte proliferation and activation, and hematopoietic stem cell differentiation. CD90 has been shown to interact with CD45.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W18103A reacts to both the CD90.1 and CD90.2 variants. Clone W18103A can reduce CD3+ cell numbers during in vitro incubation with dose-dependent manner.
RRID: AB_3662366 (BioLegend Cat. No. 166703)
Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells
Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Cell Type: Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neurons, T cells, Thymocytes
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press. Craig W, et al. 1993. J Exp Med. 177:1331-42. Mayani H, et al. 1994. Blood. 83:2410-7.
Gene ID: 21838
UniProt: View information about CD90 on UniProt.org
Clone: W18103A
Regulatory Status: RUO
Other Names: Thy-1, Thy1
Isotype: Rat IgG2c, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.