Biolegend, 167105, Brilliant Violet 421™ anti-mouse CD7 Antibody, 50μg

CD7, also known as Leu-9, is an approximately 40 kD glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily. The extracellular domain of mature mouse CD7 shares 43% and 81% amino acid sequence similarity with human and rat CD7, respectively.  Mouse CD7 mRNA has been found in thymus, spleen, bone marrow, and small intestine. Both the ligand and function of mouse CD7 are not yet clear.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD7-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_3698936 (BioLegend Cat. No. 167105)
Structure: Type I transmembrane glycoprotein; Ig superfamily
Distribution: Mouse CD7 mRNA exists in thymus, spleen, bone marrow, and small intestine
Cell Type: Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Yoshikawa K, et al. 1993. Immunogenetics. 37:114-9. Haynes BF, et al. 1999. Crit Rev Immunol. 19:331. Bonilla FA, et al. 1997. Int Immunol. 9:1875.
Gene ID: 12516
UniProt: View information about CD7 on UniProt.org
Clone: S23010B
Regulatory Status: RUO
Isotype: Rat IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.