Biolegend, 167302, Purified anti-mouse CCL17 (TARC) Recombinant Antibody, 100μg

CCL17 was initially isolated from phytohemagglutinin-stimulated PBMCs. CCL17 is constitutively expressed in thymus and, upon activation, in several cell types. CCL17-mediated recruitment of Th2 cells and CLA + CD4 + T cells and plays a key role in allergic diseases like atopic dermatitis, allergic asthma, allergic rhinitis, and allergic contact dermatitis. In addition, CCL17 has been detected in idiopathic pulmonary fibrosis. Also, CCL17 and CCL22 secreted by DCs seem to mediate the recruitment of Tregs to sites of inflammation in patients with chronic hepatitis. In addition, CCL17 is present in advanced human and mouse atherosclerosis, and CCL17+ DCs accumulate in atherosclerotic lesions. CCL17 expressed by DCs limit the expansion of Tregs. CCL17 is upregulated by a combination of cytokines (TNFa, IL-4, and IFNγ) in bronchial epithelial cells; TGFβ1, TNFα, and IFNγ in the human keratinocyte cell line; and histamine and prostaglandin E2P in immature DC.
100μg
Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Rat
Immunogen: Recombinant mouse CCL17 (TARC)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance in each application.
RRID: AB_3683194 (BioLegend Cat. No. 167302)
Structure: Chemokine
Distribution: Upon activation, CCL17 is expressed by PBMCs, monocytes, dendritic cells, bronchial epithelial cells, smooth muscle cells, keratinocytes, and fibroblasts.
Function: CCL17 chemoattracts T cells, Th2 cells, and CLA+ CD4+ T cells. It plays a major role in allergic diseases. CCL17 can be upregulated by IL-4 in T cells and by a combination of cytokines in different cells.
Interaction: T cells, Th2 cells, CLA+ CD4+ T cells, T (reg) cells, NK cells, NKT cells, and macrophages.
Ligand/Receptor: CCR4 (CD194)
Cell Type: Dendritic cells, Epithelial cells, Fibroblasts, Monocytes
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Imai T, et al. 1997. J. Biol. Chem. 272:15036. McIlroy A, et al. 2006. Immunology. 117:507. Yogo Y, et al. 2009. Respir. Res. 10:80. Stutte S, et al. 2010. P. Natl. Acad. Sci USA. 107:8736. Oo YH, et al. 2010. J. Immunol. 184:2886. Weber C, et al. 2011. J. Clin. Invest. 121:2898.
Gene ID: 20295
UniProt: View information about CCL17 on UniProt.org
Clone: A20096E.Rec
Regulatory Status: RUO
Other Names: CC chemokine TARC, C-C motif chemokine 17, CCL17, chemokine (C-C motif) ligand 17, small inducible cytokine subfamily A (Cys-Cys), member 17, Small-inducible cytokine A17, T cell-directed CC chemokine, TARC, Thymus and activation-regulated chemokine
Isotype: Rat IgG2b, κ