Biolegend, 167504, PE anti-mouse CCL22 Antibody, 100μg

CCL22, also known as macrophage-derived chemokine (MDC), is a conventional CC chemokine that is expressed constitutively under homeostatic conditions and can be induced in several cell types upon activation. Mouse CCL22 is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA is also detectable in lung, unstimulated spleen cells, lymph node cells, and in thymocytes. Constitutive expression of CCL22 is mediated by T cell–derived GM-CSF. The only known receptor for CCL22 is CCR4, which is mainly expressed by activated Th2-biased CD4+ T cells and by regulatory T cells. CCL22 displays chemotactic activity for natural killer cells, chronically activated T lymphocytes, monocytes, and dendritic cells, and may play a role in recruiting activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CCL22 (aa25-92)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance in each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3683197 (BioLegend Cat. No. 167503) AB_3683197 (BioLegend Cat. No. 167504)
Structure: Chemokine
Distribution: Macrophages, NK cells, thymic epithelial cells and intestinal epithelial cells
Function: CCL22 induces the migration of CCR4 + Th2 cells, Tregs, DC, and NK cells. It is induced by LPS and CD40 antibody in macrophages. It is upregulated by Th2 cytokines (IL-4 and IL-5) and downregulated by Th1 cytokines (IFNγ).
Interaction: Dendritic cells, NK cells, Th2 cells, T regs, CLA+ skin-homing memory/effector T cells
Ligand/Receptor: CCR4
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Kanazawa N, et al. 1999. Eur. J. Immunol. 29: 1925–32 Schaniel C, et al. 1998. J. Exp. Med. 188:451. Andrew DP, et al. 1998. J. Immunol. 161(9):5027-38. Ghadially H, et al. 2005. J Immunol. 174(9):5620–29 Godiska R, et al. 1997. J. Exp. Med. 185:1595. Chang, M-S. et al. 1997. J. Biol. Chem. 272:25229 Piseddu I, et al. 2020. J Immunol. 205(8): 2056–65.
Gene ID: 20295
UniProt: View information about CCL22 on UniProt.org
Clone: W20219K
Regulatory Status: RUO
Other Names: Macrophage derived cytokine (MDC), Small inducible cytokine subfamily A, member 22 (Scya22), stimulated T cell chemotactic protein (STCP-1), activated B cell-derived chemokine-1 (ABCD-1) DC, B cell-derived chemokine (DC/B-CK)
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.