Biolegend, 200202, Purified anti-rat CD80 Antibody, 500μg

CD80 is a 60 kD member of the immunoglobulin superfamily also known as B7-1, B7, and BB1. It is expressed on antigen presenting cells and activated T and B cells, and is a ligand for CD28 and CD152 (CTLA-4). CD80/CD28 interactions are important for T and B cell co-stimulation, while CD80/CD152 lead to the inhibition of T cell responses. The 3H5 antibody can block the co-stimulatory activity of CD80.
500μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of ligand binding1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200208).
Application References(PubMed link indicates BioLegend citation): Maeda, K., et al. 1997. Int. Immunol. 9:993. (IP, Block) Bluestone, J.A., 1995. Immunity 2:555. (IP, Block) Damoiseaux, J.G., et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC) Ho, CH., et al. 2007. J. Virol. 81:4837. PubMed Ho, C., et al. 2009. Carcinogenesis 30:1443. PubMed
Product Citations: Ho C, et al. 2009. Carcinogenesis. 30:1443. PubMed Ho C, et al. 2007. J Virol. 81:4837. PubMed
RRID: AB_313843 (BioLegend Cat. No. 200202)
Structure: Ig superfamily, 60 kD
Distribution: Antigen presenting cells, activated B cells and T cells
Function: T and B cell costimulation
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: Antigen-presenting cells, B cells, T cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Linsley, P.S., et al. 1991. J. Exp. Med. 174:561. 3. Salomon, B., et al. 2001. Ann. Rev. Immunol. 19:225.
Gene ID: 25408
UniProt: View information about CD80 on UniProt.org
Clone: 3H5
Regulatory Status: RUO
Other Names: B7-1, B7, BB1, Ly-53
Isotype: Mouse IgG1, κ