Biolegend, 201005, Brilliant Violet 421™ anti-rat CD68 Recombinant Antibody, 50μg

CD68, also known as macrosialin, is a 110 kD integral membrane glycoprotein from the lysosomal-associated membrane protein (LAMP) family. It is predominantly expressed on the intracellular lysosomes of monocytes and macrophages, and is considered a pan-macrophage marker in the rat. CD68 may have functions relating to phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions.
50μg
Verified Reactivity: Rat
Reported Reactivity: Bovine
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone QA20A71 can completely block the binding of parental clone to target cells. Both Cyto-Fast™ Fix/Perm Buffer Set and True-Nuclear™ Transcription Factor Buffer Set work well. This clone does not react to human and mouse.
Structure: Lysosome-associated membrane glycoprotein; single-pass type I membrane protein, 110 kD
Distribution: Macrophages, Langerhans cells, and at low levels on dendritic cells
Function: May involved in cell-cell interaction or cell-ligand interaction
Cell Type: Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References: Dijkstra CD, et al. 1985. Immunology. 54:589-99. Damoiseaux JG, et al. 1994. Immunology. 83:140-7.
Gene ID: 287435
UniProt: View information about CD68 on UniProt.org
Clone: QA20A71
Regulatory Status: RUO
Other Names: Macrosialin, GP110, LAMP4, Scavenger Receptor D1 (SR-D1)
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.