Biolegend, 250502, Purified anti-rat CD38 Antibody, 100μg

CD38 is a 45 kD type II transmembrane ADP-ribosyl cyclase expressed on early hematopoietic precursors and leukocytes, hepatic stellate cells, astrocytes and epithelial cells. CD38 expression can be upregulated upon cell activation. CD38 catalyzes the production of cyclic APD ribose, which is important in Ca 2+ release. CD38 induces production of proinflammatory cytokines and proliferation. The ligand for CD38 is CD31. Impairment in CD38 expression is associated with immunological and behavioral disorders.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIHC-F - VerifiedWB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry1 and Western blotting1.
Application References(PubMed link indicates BioLegend citation): March S, et al. 2007. Am. J. Pathol. 170:176. (IHC, WB)
Product Citations: Ardiana M, et al. 2022. F1000Res. 10:917. PubMed Boslett J, et al. 2018. Am J Physiol Cell Physiol. 314:C297. PubMed
RRID: AB_2562587 (BioLegend Cat. No. 250502)
Structure: CD38 is a 45 kD type II transmembrane protein.
Distribution: Early hematopoietic precursors and leukocytes, astrocytes, hepatic stellate cells, and epithelial cells; upregulated upon activation.
Function: CD38 catalyzes the production of cyclic APD ribose, which is important in Ca2+ release. CD38 induces production of proinflammatory cytokines and proliferation.
Ligand/Receptor: CD31, hyaluronic acid
Cell Type: Astrocytes, B cells, Dendritic cells, Epithelial cells, Leukocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, MHC Antigens
Antigen References: 1. Ota H, et al. 2012. Life Sci. 90:206. 2. Masuda W, et al. 2011. Arch. Biochem. Biophys. 2:131. 3. Khoo KM, et al. 1998. Biochem. Mol. Biol. Int. 44:841. 4. Prasad GS, et al. 1996. Nat. Struct. Biol. 3:957.
Gene ID: 25668
UniProt: View information about CD38 on UniProt.org
Clone: 14.27
Regulatory Status: RUO
Other Names: T10 , ADP-ribosyl cyclase
Isotype: Mouse IgG2b, κ