Biolegend, 260100, GMP FITC anti-Cytochrome c Antibody, 100tests

Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat cyt c-OVA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 200 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.
Application References(PubMed link indicates BioLegend citation): Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134. Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143. Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF) Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP) Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF) She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Heme binding domain; 15 kD
Distribution: Mitochondrial intermembrane space
Function: Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
Interaction: Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
Molecular Family: Mitochondrial Markers
Antigen References: Liu X, et al. 1996. Cell. 86:147. Li P, et al. 1997. Cell. 91:479. Zhang Z, et al. 2003. Gene 312:61. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
Gene ID: 1355
UniProt: View information about Cytochrome c on UniProt.org
Clone: 6H2.B4
Other Names: Cyt c
Isotype: Mouse IgG1, κ