Biolegend, 260162, GMP PerCP/Cyanine5.5 anti-human CD3 Antibody, 100tests

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 100 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.
Application References(PubMed link indicates BioLegend citation): Kan EA, et al. 1983. J. Immunol. 131:536. Wood GS, et al. 1985. Am. J. Pathol. 120:371. Van Dongen JJM, et al. 1988. Blood 71:603. (WB) Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC) Carbone A, et al. 1999. Blood 93:2319. (IHC) Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF) Rutjens E, et al. 2007. J. Immunol. 178:1702. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, during thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID: 916
UniProt: View information about CD3 on UniProt.org
Clone: SK7
Workshop: HCDM listed
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ