Biolegend, 260180, GMP PerCP/Cyanine5.5 anti-human CD4 Antibody, 100tests

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 100 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Evans RL, et al. 1981. Immunol. 78:544 Arno A et al. 1999. J. Infect. Dis. 180:56 Muech M, et al. 1997. Blood 89:1364 Wang L, et al. 2012. Cytometry A. 81:567. PubMed
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D et al. 1996. Immunol. Today 17:476. 2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920
UniProt: View information about CD4 on UniProt.org
Clone: SK3
Other Names: T4, Leu3a
Isotype: Mouse IgG1, κ