Biolegend, 260246, GMP APC anti-human CD45RA Antibody, 100tests

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 6 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block) Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F) Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P) Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed Palendira U, et al. 2008. Blood (FC) PubMed Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed Canque B, et al. 2000. Blood 96:3748. (ICC) Imanguli MM, et al. 2009. Blood 13:3620. (ICC) Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG) Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution: B cells, naïve T cells, monocytes
Function: Enhances TCR and BCR signaling
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID: 5788
UniProt: View information about CD45RA on UniProt.org
Clone: HI100
Workshop: IV N906
Other Names: GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype: Mouse IgG2b, κ