Biolegend, 260262, GMP APC anti-human CD38 Antibody, 100tests

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 100 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and spatial biology (IBEX)10,11.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Dieu M. 1998. J. Exp. Med. 188:373. Esser M, et al. 2001. J. Virol. 75:6173. Jeannin P, et al. 1999. J. Immunol. 162:2044. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. van der Voort R, et al. 1997. J. Exp. Med. 185:2121. (IHC) Bende RJ, et al. 2003. Am. J. Pathol. 162:105. Lehner M, et al. 2008. J. Leukoc. Biol. 83:883. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
Distribution: T cells, B cells, NK, myeloid, plasma, and dendritic cells
Function: Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
Ligand/Receptor: CD31, hyaluronic acid
Cell Type: B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Ferrero E, et al. 1999. J. Leukoc. Biol. 65:151. 2. Lund F, et al. 1995. Immunol. Today 16:469.
Gene ID: 952
UniProt: View information about CD38 on UniProt.org
Clone: HIT2
Workshop: III 155
Other Names: T10, ADP-ribosyl cyclase
Isotype: Mouse IgG1, κ