Biolegend, 280302, Purified Streptavidin, 250μg

Streptavidin is a 52 kD tetrameric protein obtained from Streptomyces avidinii . It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10 -14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.
250μg
Formulation: Phosphate-buffered solution containing 0.09% sodium azide.
Concentration: 1.0 mg/ml
Storage & Handling: Store between 2°C and 8°C.
Application: FC - Quality tested ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house
Recommended Usage: To coat one 96-well plate dilute 24 µL of Purified Streptavidin into 11.976ml of 1X Coating Buffer (Cat. No. 421701, Coating Buffer 5X concentrate). Final concentration in the coated plate is 2 µg/mL. This recommendation is to perform the protocol for HLA class I ELISA to evaluate Flex-T™ peptide exchange. For other applications the reagent needs to be optimized.
Product Citations: Wu C, et al. 2022. ACS Nano. 16:1025. PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Wu C, et al. 2020. J Am Chem Soc. 142:12314. PubMed
Structure: 52 kD tetrameric protein.
Distribution: Streptavidin binds to biotin with high affinity.
Ligand/Receptor: Biotin.
Antigen References: 1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343. 2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093. 3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135. 4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113. 5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.
Gene ID: NA
Regulatory Status: RUO
Other Names: Streptavidin-PURE, SAV-PU, SAV