Biolegend, 300009, FITC anti-human LAP (TGF-β1) Antibody, 25tests

TGF-β1 (transforming growth factor β1) is a multifunctional cytokine which regulates cellular proliferation and differentiation. It is ubiquitously expressed by many types of cells. Platelets express high level of TGF-β. TGF-β is synthesized as a large protein precursor and then secreted as a complex of TGF-β and LAP (latency-associated peptide), in which LAP noncovalently associates with the dimeric mature TGF-β to prevent its activity. TGF-β requires activation before it binds to its receptors and exerts functions. It has been reported that LAP-TGF-β binds to the integrins αvβ1, αvβ6, αvβ8, and α8β1 through RGD domain. TGF-β plays important roles in control proliferation and differentiation of epithelial cells, endothelial cells, fibroblasts, neurons, osteoclasts, and osteoblasts. TGF-β is believed to be important in the regulation of the development of Treg, Th17, and Th9 cells. A recent study has shown that LAP is an activated Treg surface marker.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human TGF-β1 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone S2006A can completely block the binding of clones TW4-2F8 and TW4-6H10 on target cells.
Product Citations: Bode K, et al. 2024. Front Immunol. 1403752:15. PubMed
RRID: AB_2894447 (BioLegend Cat. No. 300009) AB_2894447 (BioLegend Cat. No. 300010)
Structure: About 400 amino acids, N-terminal signal peptide is required for secretion from a cell, a pro-region (LAP) and C-terminal region that becomes the mature TGF-β molecule following its release from the pro-region by proteolytic cleavage.
Distribution: Ubiquitously expressed by many kinds of cells
Function: Multifunctional cytokine, regulates cells proliferation, and differentiation
Ligand/Receptor: TGF-βRI, -RII, -RIII, CD105, LTBP, integrins
Cell Type: B cells, Dendritic cells, T cells, Tregs
Biology Area: Adaptive Immunity, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Inhibitory Molecules, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Yi JJ, et al. 2010. Cell. 142:144. Tran DQ, et al. 2009. Blood. 113:5125. Lu M, et al. 2002. J Cell Sci. 115:4641. Khalil N. 1999. Microbes Infect. 1:1255.
Gene ID: 7040
UniProt: View information about LAP on UniProt.org
Clone: S20006A
Regulatory Status: RUO
Other Names: Latency Associated Peptide (LAP), Transforming growth factor beta 1 (TGF-b1), TGFB1, DPD1, TGF-beta1
Isotype: Mouse IgG1, κ