Biolegend, 300604, Biotin anti-human CD5 Antibody, 100μg

CD5 is a 67 kD single chain type I glycoprotein also known as Leu-1, Ly-1 and T1. It is a member of the scavenger receptor superfamily found on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-CLL), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions upon binding to ligands such as CD72.
100μg
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Capuchin Monkey, Common Marmoset, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, it is recommended to use at ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting2 and immunohistochemical staining of acetone-fixed frozen sections2,5.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York. Renaudineau Y, et al. 2005. Blood 106:2781. (WB IHC) Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. Saliba AE, et al. 2010. P. Natl. Acad. Sci. USA 107:14524. PubMed Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
RRID: AB_314090 (BioLegend Cat. No. 300604)
Structure: Scavenger receptor superfamily, 67 kD
Distribution: T cells, thymocytes, B cell subset, B cell CLL, peripheral blood dendritic cells
Function: Modulates TCR, BCR signaling, thymocyte maturation, T-B cell interaction
Ligand/Receptor: CD72
Cell Type: B cells, Dendritic cells, Leukemia, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Kipps T. 1988. Adv. Immunol. 47:117. 2. Resnick D, et al. 1993. Trends Biochem. Sci. 19:5. 3. Wood GS, et al. 1992. Am. J. Pathol. 14:789.
Gene ID: 921
UniProt: View information about CD5 on UniProt.org
Clone: UCHT2
Regulatory Status: RUO
Workshop: III 518
Other Names: Leu-1, Ly-1, T1, Tp67
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.