Biolegend, 301643, Brilliant Violet 785™ anti-human CD11c Antibody, 25tests

CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin α X and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
25tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Chimpanzee, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York. Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC) Ottonello L, et al. 1999. Blood 93:3505. Metelitsa LS, et al. 2002. Blood 99:4166. Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. PubMed Ihanus E, et al. 2007. Blood 109:802-810. Gurer C, et al. 2008. Blood 112:1231. PubMed Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC)
Product Citations: Upadhyay AA, et al. 2023. Nat Commun. 14:1914. PubMed Newton HS, et al. 2024. Methods Mol Biol. 269:2789. PubMed Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed Pozzetto B, et al. 2021. Nature. 600:701. PubMed Hoang TN, et al. 2021. Cell. 184:460. PubMed Hoang TN, et al. 2020. bioRxiv. . PubMed Mishra A, et al. 2018. J Allergy Clin Immunol. 142:1066. PubMed Charmetant X, et al. 2022. Sci Transl Med. :eabl6141. PubMed Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
RRID: AB_2565778 (BioLegend Cat. No. 301643) AB_2565779 (BioLegend Cat. No. 301644)
Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Distribution: Myeloid, dendritic cells, NK cells, B cells and T cell subsets
Function: Adhesion, CTL killing
Ligand/Receptor: CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
Cell Type: B cells, Dendritic cells, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Petty H. 1996. Immunol. Today 17:209. 2. Springer T. 1994. Cell 76:301. 3. Ihanus E, et al. 2007. Blood 109:802-810.
Gene ID: 3687
UniProt: View information about CD11c on UniProt.org
Clone: 3.9
Regulatory Status: RUO
Workshop: III NL707
Other Names: Integrin αX subunit, CR4, p150, ITGAX
Isotype: Mouse IgG1, κ