Biolegend, 302025, Alexa Fluor® 700 anti-human CD16 Antibody, 25μg

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
25μg
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PMN cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP) Hoshino S, et al. 1991. Blood 78:3232. (Stim) Tamm A, et al. 1996. Immunol. 157:1576. (Block) Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC) Holl V, et al. 2004. J. Immunol. 173:6274. (Block) Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block) Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Rout N, et al. 2010. PLoS One 5:e9787. (FC) Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC) Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed Wu Z, et al. 2013. J. Virol. 87:7717. PubMed Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG) Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
Product Citations: Lee HS, et al. 2023. Biol Pharm Bull. 46:542. PubMed Carre C, et al. 2021. iScience. 24:102970. PubMed Diny NL, et al. 2022. J Exp Med. 219: . PubMed Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed Cluff E, et al. 2022. Cancer Immunol Immunother. . PubMed Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed Koutsakos M, et al. 2021. Cell Reports Medicine. 2(3):100208. PubMed de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed Veenhuis RT, et al. 2021. JCI Insight. 6:. PubMed Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed Chalan P, et al. 2015. PLoS One. 10: 0132436. PubMed Ma YV, et al. 2021. MAbs. 13:2003281. PubMed Poznanski SM, et al. 2018. Cancer Immunol Res. 1.065277778. PubMed Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed Miao L, et al. 2021. Clin Transl Med. 11:e395. PubMed Nguyen THO, et al. 2021. Immunity. 54:1066. PubMed Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed Lusty E, et al. 2017. Mol Immunol. 10.1016/j.molimm.2017.06.025. PubMed Forsberg MH, et al. 2021. Stem Cell Res Ther. 12:459. PubMed Vaccari M, et al. 2013. J Virol. 87:3526. PubMed Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed Pham TNQ, et al. 2020. Cell Reports. 29(9):2770-2782.e5.. PubMed Georg P, et al. 2022. Cell. 185:493. PubMed NULL, et al. 2022. Cell. 185:916. PubMed Wimmers F, et al. 2021. Cell. 184:3915. PubMed Pate K, et al. 2015. J Infect Dis. 212: 1387 - 1396. PubMed Lee GR, et al. 2021. JCI Insight. 6:. PubMed O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed Vanoni G, et al. 2021. eLife. 10:00. PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed Connors J, et al. 2022. Geroscience. :. PubMed Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed Chowdhury A, et al. 2015. J Virol. 89: 8677-8686. PubMed
RRID: AB_493748 (BioLegend Cat. No. 302025) AB_2278418 (BioLegend Cat. No. 302026)
Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution: NK cells, activated monocytes, macrophages, neutrophils
Function: Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor: Aggregated IgG, IgG-antigen complex
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275. 2. Stroncek D, et al. 1991. Blood 77:1572. 3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.
Gene ID: 2214
UniProt: View information about CD16 on UniProt.org
Clone: 3G8
Regulatory Status: RUO
Workshop: V NK80
Other Names: FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype: Mouse IgG1, κ
Q: Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?
A: Yes