Biolegend, 302606, PE anti-human CD25 Antibody, 100tests

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4 + T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
100tests
Verified Reactivity: Human
Reported Reactivity: Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications include: immunocytochemistry3.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations: Riner D, et al. 2016. PLoS Negl Trop Dis. 10:e0005180. PubMed Tang M, et al. 2019. Biomed Res Int. 2019:1050285. PubMed Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed Yang M, et al. 2019. Cell Physiol Biochem. 52:1178. PubMed Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed Hu Y, et al. 2023. Front Immunol. 14:1128423. PubMed Pfister F, et al. 2023. Methods Mol Biol. 2644:331. PubMed Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed Freeman ZT, et al. 2020. J Clin Invest. 130:1405. PubMed Miao L, et al. 2020. Theranostics. 0.7625. PubMed Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed Savage TM, et al. 2018. JCI Insight. 3:. PubMed Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed Saadati S, et al. 2020. Avicenna J Med Biotechnol. 0.591666667. PubMed Wei H, et al. 2021. Malar J. 20:89. PubMed Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed Vinogradova EV, et al. 2021. STAR Protoc. 2:100458. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Goletz C, et al. 2018. Front Immunol. 9:1614. PubMed de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed Lopatina T, et al. 2018. Sci Rep. 8:17458. PubMed Ramien C, et al. 2020. Cell Reports. 29(4):810-815. PubMed Pothlichet J, et al. 2022. Front Immunol. 13:824746. PubMed Bellini N, et al. 2022. iScience. 25:105234. PubMed Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed James E, et al. 2016. PLoS Pathog. 12: 1005375. PubMed Chen Y, et al. 2012. PLoS One. 7:e47190. PubMed Soodgupta D, et al. 2019. Cell Rep. 29:829. PubMed Meng S, et al. 2018. Mol Med Rep. 18:4247. PubMed Zhang W, et al. 2012. J Biol Chem. 287:34157. PubMed Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed Robertson N, et al. 2020. J Biol Chem. . PubMed Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed Meléndez AV, et al. 2022. Cell Mol Life Sci. 79:513. PubMed Lee JM, et al. 2022. Lupus Sci Med. 9:. PubMed Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed Wu D, et al. 2020. Biomark Res. 8:3. PubMed Chen Y, et al. 2021. Transl Lung Cancer Res. 10:2193. PubMed Erdem A, et al. 2022. Nat Commun. 13:1105. PubMed You M, et al. 2021. Nat Cell Biol. 23:620. PubMed Carnevale J, et al. 2022. Nature. 609:174. PubMed Zhou Y, et al. 2016. J Leukoc Biol. 100: 1201 - 1211. PubMed Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed Shen X, et al. 2021. Front Immunol. 12:710750. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Mengrelis K, et al. 2021. Pediatr Transplant. 25:e13930. PubMed Reichwald K, et al. 2014. PLoS One. 9:105627. PubMed Luo X, et al. 2015. J Biol Chem. 290: 28675 - 28682. PubMed Batikan O, et al. 2022. J Coll Physicians Surg Pak. 32:1004. PubMed Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed Keefe RC, et al. 2021. Sci Rep. 11:14933. PubMed Tian M, et al. 2021. Elife. 10:. PubMed Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed Imura Y, et al. 2020. JCI Insight. :5. PubMed Vinogradova EV, et al. 2020. Cell. 182(4):1009-1026.e29. PubMed Han L, et al. 2014. J Biol Chem. 289:25546. PubMed Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed Zhang B, et al. 2020. Cell Chemical Biology. 28(1):60-69.e7. PubMed Ferraro A, et al. 2014. Proc Natl Acad Sci U S A. 111:1111. PubMed Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed Ajith A, et al. 2019. FASEB J. 33:5220. PubMed Wang L, et al. 2018. Oncol Lett. 15:8635. PubMed Cerny D, et al. 2016. J Allergy Clin Immunol. 138:1709-1712. PubMed Fierle JK, et al. 2021. Cell Reports Medicine. 2(8):100362. PubMed Robertson N, et al. 2020. J Biol Chem. 295:18436. PubMed Welch JL, et al. 2020. FEBS Lett. 594:695. PubMed Wang AS, et al. 2020. Molecular Cell. 79(2):221-233.e5.. PubMed Kim N, et al. 2020. Nat Commun. 2.045138889. PubMed Hui Z, et al. 2022. Cell Death Dis. 13:607. PubMed Högelin KA, et al. 2021. iScience. 24:103078. PubMed Choe JH, et al. 2021. Sci Transl Med. 13:. PubMed
RRID: AB_314275 (BioLegend Cat. No. 302605) AB_314276 (BioLegend Cat. No. 302606)
Structure: Type I transmembrane glycoprotein, 55 kD
Distribution: Activated T cells and B cells, monocytes/macrophages, Treg
Function: Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor: IL-2
Cell Type: B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
Gene ID: 3559
UniProt: View information about CD25 on UniProt.org
Clone: BC96
Regulatory Status: RUO
Workshop: V T-072
Other Names: Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.