Biolegend, 302978, Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD28 SF Antibody, 1mg

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major costimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation. GMP Ultra-LEAF™ Biotin anti-human CD28 SF Antibody was GMP manufactured under serum-free conditions including serumfree hybridoma cell culture, without additional animal or humanderived materials or preservatives. This antibody contains ultra-low levels of endotoxin (<0.01EU/μg of protein), is filtered through a 0.1 μm membrane, and is tested negative for mycoplasma and microbial growths. GMP Ultra-LEAF™ Biotin anti-human CD28 SF Antibody is for research use only. Please inquire any additional application right by contacting BioLegend. BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
1mg
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: 0.1 μm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: 1.0 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested Activation - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: GMP Ultra-LEAF™ Biotin anti-human CD28 SF Antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.1 μm filtered) is recommended for highly sensitive assays. The antibody was GMP manufactured under serum-free conditions including serum-free hybridoma cell culture, without additional animal or human-derived materials or preservatives. This antibody is intended for flow cytometry and for research use only.
Additional Product Notes: This antibody is intended for flow cytometry and activation/expansion of T cells for research use only. Please inquire any additional application right by contacting BioLegend. For plate coating protocols, CD28.2 antibody (Cat. No. 302977/302978) can be used at 1 μg/mL in PBS in combination with 1 μg/mL of Biotin anti-human CD3 antibody (Cat. No. 317358) to efficiently activate T cells. Plates should be coated for 2 hours in a tissue culture incubator or overnight at 4°C. For soluble antibody activation protocols, we recommend 10 μg/mL of antihuman CD3 (Cat. No. 317358) antibody and 5 μg/mL of anti-human CD28 antibody (Cat. No. 302977/302978).
Disclaimer: BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution: Mature T cells, thymocytes, NK cell subsets, plasma cells, EBVpositive B cells
Function: T cell costimulation
Ligand/Receptor: CD80/CD86
Cell Type: B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. June CH, et al. 1994. Immunol Today. 15:321-31. Linskey PS and Ledbetter JA. 1993. Annu Rev Immunol. 11:191-212.
Gene ID: 940
UniProt: View information about CD28 on UniProt.org
Clone: CD28.2
Other Names: T44, Tp44
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.