Biolegend, 303110, Alexa Fluor® 488 anti-human CD31 Antibody, 100tests

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.
100tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF) Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block) Nagano M, et al. 2007. Blood 110:151. (FC) PubMed MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Jeon NL, et al. 2020. Biotechnol Bioeng. 117:748. PubMed Antonyshyn JA, et al. 2022. Biomater Biosyst. 6:100049. PubMed Kameishi S, et al. 2023. Sci Rep. 13:4421. PubMed Smith MH, et al. 2023. Nat Immunol. . PubMed Benjamin DI, et al. 2022. Cell Metab. 34:902. PubMed Zachara M, et al. 2022. EMBO J. 41:e108206. PubMed Wei X, et al. 2022. Mol Cell. 82:1225. PubMed Hu-Lowe D, et al. 2011. Cancer Res. 71:1362. PubMed Chen W, et al. 2021. Nat Microbiol. 6:455. PubMed Nishino K, et al. 2021. Sci Rep. 11:8406. PubMed Bradfield P, et al. 2016. PLoS One. 11: 0159679. PubMed Ajit A, et al. 2020. J Biomed Mater Res B Appl Biomater. 108:2807. PubMed Qi J, et al. 2022. Nat Commun. 13:1742. PubMed Sarkar TJ, et al. 2020. Nat Commun. 11:1545. PubMed Judson RN et al. 2018. Cell stem cell. 22(2):177-190 . PubMed Hallett JM, et al. 2022. Cell Stem Cell. 29:355. PubMed Quarta M, et al. 2016. Nat Biotechnol. 10.1038/nbt.3576. PubMed Khan OF, et al. 2018. Sci Adv. 4:eaar8409. PubMed Harvestine JN, et al. 2018. J Mater Chem B. 6:4104. PubMed Park D, et al. 2021. Sci Rep. 11:19986. PubMed Hedegaard CL, et al. 2020. Sci Adv. 6:00. PubMed Harvestine JN, et al. 2020. Sci Adv. 6:eaay2387. PubMed Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed Ajit A, et al. 2019. Tissue Eng Part C Methods. 25:148. PubMed Xu J, et al. 2017. Clin Cancer Res. 23:4482. PubMed Meng YM, et al. 2018. Oncoimmunology. 7:e1408745. PubMed Tsukada Y, et al. 2021. Sci Rep. 0.975694444. PubMed Kumashiro Y, et al. 2015. Small. 6: 681 - 687. PubMed Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed Antonyshyn JA, et al. 2021. Commun Biol. 4:1205. PubMed
RRID: AB_493075 (BioLegend Cat. No. 303109) AB_493074 (BioLegend Cat. No. 303110)
Structure: Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution: Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset
Function: Cell adhesion, signal transduction
Ligand/Receptor: CD38
Cell Type: Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: DeLisser H, et al. 1994. Immunol. Today 15:490. Newman P, 1997. J. Clin. Invest. 99:3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
Gene ID: 5175
UniProt: View information about CD31 on UniProt.org
Clone: WM59
Regulatory Status: RUO
Workshop: V P025
Other Names: PECAM-1, EndoCAM
Isotype: Mouse IgG1, κ