Biolegend, 303439, APC/Fire™ 750 anti-human CD33 Antibody, 25tests

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
25tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human myeloid leukaemia cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB) Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
RRID: AB_2814132 (BioLegend Cat. No. 303439) AB_2814133 (BioLegend Cat. No. 303440)
Structure: Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution: Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
Function: Adhesion and lectin activity
Ligand/Receptor: Sugar chains containing sialic acid
Cell Type: B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: 1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. 2. Freeman S, et al. 1995. Blood 85:2005.
Gene ID: 945
UniProt: View information about CD33 on UniProt.org
Clone: WM53
Regulatory Status: RUO
Workshop: IV M-505
Other Names: Siglec-3, gp67, p67
Isotype: Mouse IgG1, κ