Biolegend, 303651, PE Recombinant Protein L, 50tests

Protein L was initially isolated from bacteria Peptostreptococcus magnus cell wall, and now is prepared as recombinant protein. Protein L has been found to have a high affinity for Ig κ light chains. This unique ability of protein L to bind to the κ light chains of antibodies makes it valuable in detection and purification of antibodies and antibody fragments that contain kappa light chains but not the Fc region, such as antigen-binding VL domains and Fv fragments prepared from monoclonal antibodies. It is also used to detect chimeric antigen receptor (CAR) expressing cells.
50tests
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: For multi-color flow cytometry analysis, cells should be stained with Protein L first, and washed; costaining reagent(s) need to be applied as the second step.
Cell Type: B cells
Biology Area: Cell Biology, Immuno-Oncology, Immunology
Antigen References: Björck L. 1988. J Immunol. 140:1194-7. Akerström B and Björck L. 1989. J Biol Chem. 264:19740-6.
Gene ID: NA
UniProt: View information about Protein L on UniProt.org
Regulatory Status: RUO
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.