Biolegend, 304601, Purified anti-human CD56 (NCAM) Antibody, 25μg

CD56 is a single transmembrane glycoprotein also known as N-CAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
25μg
Verified Reactivity: Human
Reported Reactivity: Cow, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KG-1 human acute myelogenous leukemia cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-P, IP, WB, - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, and Western blotting (non-reducing).
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Product Citations: Klawitter M, et al. 2022. Int J Mol Sci. 23:. PubMed Korn MA, et al. 2020. J Immunol. 205:2595. PubMed de Groot N, et al. 2021. Porcine Health Manag. 7:60. PubMed Mann ER, et al. 2020. Sci Immunol. :5. PubMed Zhan X, et al. 2020. Journal of Experimental Medicine. 217(5):e20191340. PubMed Zeniou M, et al. 2015. PLoS One. 10: 0134793. PubMed Peng T, et al. 2021. Front Immunol. 12:735643. PubMed De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
RRID: AB_314443 (BioLegend Cat. No. 304601) AB_314444 (BioLegend Cat. No. 304602)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein, 175-185 kD
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparin sulfate
Cell Type: Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: MEM-188
Regulatory Status: RUO
Workshop: VI NK26
Other Names: NCAM, Leu-19, NKH1
Isotype: Mouse IgG2a, κ