Biolegend, 304801, Purified anti-human CD62L Antibody, 25μg

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
25μg
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Concentrated supernatant from PMA-activated human peripheral blood leukocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested WB, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 304853-304858).
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block) Jutila M, et al. 2002. J. Immunol. 169:1768. (WB) Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB) Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Product Citations: Lizcano A et al. 2017. Blood. 129(23):3100-3110 . PubMed Rodriguez L, et al. 2020. Cell Reports Medicine. 1(5):100078. PubMed Mann ER, et al. 2020. Sci Immunol. :5. PubMed Shi C, et al. 2011. J Immunol. 187:5293. PubMed Zhang Y, et al. 2020. Front Immunol. 11:1012. PubMed Syrimi E, et al. 2021. iScience. 24:103215. PubMed Tecle E, et al. 2019. J Biol Chem. 294:11910. PubMed Li G, et al. 2022. Mol Ther Oncolytics. 24:887. PubMed Lazarski CA, et al. 2020. Cytotherapy. . PubMed De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
RRID: AB_314462 (BioLegend Cat. No. 304802)
Structure: Selectin, single chain glycoprotein, 74-95 kD
Distribution: Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
Function: Leukocyte homing, leukocyte tethering, rolling
Ligand/Receptor: CD34, GlyCAM, MAdCAM-1
Cell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. Kishimoto T, et al. 1991. Blood 78:805.
Gene ID: 6402
UniProt: View information about CD62L on UniProt.org
Clone: DREG-56
Regulatory Status: RUO
Workshop: V S056
Other Names: L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
Isotype: Mouse IgG1, κ