Biolegend, 304804, FITC anti-human CD62L Antibody, 100tests

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Concentrated supernatant from PMA-activated human peripheral blood leukocytes
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 304853-304858).
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block) Jutila M, et al. 2002. J. Immunol. 169:1768. (WB) Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB) Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Product Citations: Li D, et al. 2023. Int J Oncol. 62:. PubMed Sun F, et al. 2024. Nat Commun. 615:15. PubMed Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed Antonucci L, et al. 2020. Journal of Immunology Research. 3257.929861. PubMed Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed Gadd V, et al. 2016. PLoS One. 11: 0157771. PubMed Collins DR, et al. 2021. Immunity. 54:2372. PubMed Yuen T, et al. 2010. J Virol. 84:10220. PubMed Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed Liu YG, et al. 2019. FASEB J. 33:5018. PubMed Kolchakova D, et al. 2021. Biomolecules. 11:. PubMed Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed Erdoes G, et al. 2013. PLoS One. 8:e53045. PubMed Mahajan A, et al. 2019. J Leukoc Biol. 105:1087. PubMed Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed Jiang J, et al. 2016. Sci Rep. 6: 32320. PubMed
RRID: AB_314463 (BioLegend Cat. No. 304803) AB_2564162 (BioLegend Cat. No. 304838) AB_314464 (BioLegend Cat. No. 304804)
Structure: Selectin, single chain glycoprotein, 74-95 kD
Distribution: Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
Function: Leukocyte homing, leukocyte tethering, rolling
Ligand/Receptor: CD34, GlyCAM, MAdCAM-1
Cell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. Kishimoto T, et al. 1991. Blood 78:805.
Gene ID: 6402
UniProt: View information about CD62L on UniProt.org
Clone: DREG-56
Regulatory Status: RUO
Workshop: V S056
Other Names: L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
Isotype: Mouse IgG1, κ