Biolegend, 305205, FITC anti-human CD80 Antibody, 25tests

CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.
25tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC) Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB) Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations: Tang J, et al. 2023. Research (Wash D C). 6:0033. PubMed Taylor J, et al. 2023. Front Immunol. 14:1119350. PubMed Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed Hou Y, et al. 2022. Adv Sci (Weinh). 9:e2200910. PubMed Shen Z, et al. 2022. EBioMedicine. 85:104285. PubMed Mohamed H, et al. 2023. Biomedicines. 11: . PubMed Bauer J, et al. 2022. Nat Commun. 13:6401. PubMed Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed Wang X, et al. 2021. Front Physiol. 12:644903. PubMed Miao L, et al. 2021. Clin Transl Med. 11:e395. PubMed Marn Franco JL, et al. 2020. Cell Reports. 33(13):108547. PubMed Eken A, et al. 2020. North Clin Istanb. 0.513194444. PubMed Lin H, et al. 2020. Small. 16:e2002194. PubMed Du B, et al. 2020. Scand J Immunol. 91:e12872. PubMed Liu Y, et al. 2020. Oncol Lett. 2369:20. PubMed Serhan J 2012. Blood. 120:e60. PubMed Denniston A, et al. 2012. Invest Ophthalmol Vis Sci. 53:888. PubMed Denniston A, et al. 2011. J Immunol. 186:305. PubMed Li X, et al. 2021. Cell Death Dis. 12:314. PubMed Wang J, et al. 2022. Oncoimmunology. 11:2016159. PubMed Milillo MA, et al. 2019. Front Immunol. 10:2181. PubMed Riegel K, et al. 2021. Cell Death Dis. 12:969. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Agod Z, et al. 2017. Redox Biol. 10.1016/j.redox.2017.07.016. PubMed Rusconi M, et al. 2017. Sci Rep. 5.745138889. PubMed Ronaghan NJ, et al. 2022. PLoS One. 17:e0276013. PubMed
RRID: AB_314501 (BioLegend Cat. No. 305205) AB_314502 (BioLegend Cat. No. 305206)
Structure: Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution: Activated B cells and T cells, macrophages, and dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Freeman G, et al. 1991. J. Exp. Med. 174:625. 2. Linsley P, et al. 1996. Immunity 4:535. 3. Linsley P, et al. 1991. J. Exp. Med. 174:561.
Gene ID: 941
UniProt: View information about CD80 on UniProt.org
Clone: 2D10
Regulatory Status: RUO
Workshop: VI CD80.1
Other Names: B7-1, B7, BB1
Isotype: Mouse IgG1, κ