Biolegend, 305902, Purified anti-human CD116 Antibody, 100μg

CD116 is a 70-85 kD α chain of the GM-CSF receptor. It combines with CDw131 β chain to form the high affinity GM-CSF receptor. A soluble form of CD116, which binds GM-CSF with a relatively low affinity, has been identified. In addition, an alternatively spliced form of CD116 with an altered cytoplasmic tail has been described. CD116 is expressed on various myeloid cells including monocytes, macrophages, neutrophils, eosinophils, dendritic cells and their precursors, fibroblasts, and endothelial cells. CD116 is expressed on myeloid leukemias, osteogenic sarcoma cell lines, osteoblast-like cells and breast and lung carcinoma cell lines.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human GM-CSFRα transfected COS cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP, WB, IHC-P, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting1, and immunohistochemical staining of acetone-fixed frozen tissue sections4 and paraffin-embedded tissue sections5.
Application References(PubMed link indicates BioLegend citation): Stomski FC, et al. 1998. J. Biol. Chem. 273:1192. (WB) McClure B, et al. 2001. Blood 98:3165. (IP) Guthridge MA, et al. 2004. Blood 103:820. Xiong S, et al. 2013. J. Clin. Invest. 123:4264. (IHC) Sawada H, et al. 2014. J. Exp. Med. 211:263. (IHC).
Product Citations: Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID: AB_314568 (BioLegend Cat. No. 305902)
Structure: Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70-85 kD
Distribution: Monocytes, granulocytes, dendritic cells, endothelial precursors
Function: Myeloid hematopoietic cell proliferation, differentiation
Ligand/Receptor: GM-CSF
Cell Type: Dendritic cells, Endothelial cells, Granulocytes, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Miyajima A, et al. 1993. Blood 82:1960.
Gene ID: 1438
UniProt: View information about CD116 on UniProt.org
Clone: 4H1
Regulatory Status: RUO
Other Names: GM-CSFRα chain
Isotype: Mouse IgG1, κ