Biolegend, 307206, PE anti-human CD261 (DR4, TRAIL-R1) Antibody, 100tests

DR4 is 56 kD member 10A of the TNFR superfamily (TNFRSF10A), also known as TRAIL-R1, Apo-2, and CD261. It is expressed at low levels by activated T cells and some tumors. After TRAIL engagement, DR4 (TRAIL-R1), through activation of NF-κB, induces apoptosis in the TRAIL ligated cell.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of DR4-human IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: The DJR1 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR4/TRAIL-R1 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307206) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
Application References(PubMed link indicates BioLegend citation): Uno K, et al. 2003. Blood 101:3658. Sato K, et al. 2005. J. Immunol. 174:4025. Lundqvist A, et al. 2006. Cancer Res. 66:7317. PubMed Chen KF, et al. 2011. J Pharmacol Exp Ther. 337:155. PubMed Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed Chandrasekaran S, et al. 2014. PLoS One. 9:111487. PubMed
Product Citations: Braun F, et al. 2015. PLoS One. 10:117994. PubMed Grayson KA, et al. 2021. Mol Cancer Ther. 20:833. PubMed Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed Liu S, et al. 2021. Mol Ther Oncolytics. 21:303. PubMed Magnussen G, et al. 2012. Biochem Biophys Res Commun. 420:516. PubMed Yong A, et al. 2009. Blood. 113:875. PubMed Grayson KA, et al. 2021. PLoS One. 16:e0246733. PubMed Wang Q, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.57. PubMed Greenlee JD, et al. 2021. eLife. 0.416666666666667. PubMed Yao C, et al. 2018. Autophagy. 1.854861111. PubMed Kolluri KK et al. 2018. eLife. 7 pii: e30224. PubMed Chen QZ, et al. 2020. Front Immunol. 1.202777778. PubMed Ishii Y, et al. 2021. J Biol Chem. 297:101223. PubMed Staniek J, et al. 2019. Front Immunol. 10:951. PubMed
RRID: AB_314669 (BioLegend Cat. No. 307205) AB_2287472 (BioLegend Cat. No. 307206)
Structure: TNFR superfamily, 56 kD
Distribution: Activated T cells, tumor cells
Function: Induces apoptosis
Ligand/Receptor: TRAIL
Cell Type: T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Macfarlane M. 2003. Toxicol. Lett. 139:89. 2. Baetu T, et al. 2002. Cytokine Growth Factor Rev. 13:199. 3. Degli-Esposti M. 1999. J. Leukoc. Biol. 65:535.
Gene ID: 8797
UniProt: View information about CD261 on UniProt.org
Clone: DJR1
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: TRAIL-R1, Apo-2, CD261, TNFRSF10A
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.