Biolegend, 309407, APC anti-human CD275 (B7-H2, ICOSL) Antibody, 25tests

B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human B7H2-mIg fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Kurosawa S, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:563.
Product Citations: Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed Xu C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:327. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Woodruff M, et al. 2020. medRxiv. . PubMed Tu C, et al. 2020. Oncol Rep. 44:224. PubMed Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
RRID: AB_2565556 (BioLegend Cat. No. 309407) AB_2565557 (BioLegend Cat. No. 309408)
Structure: Ig superfamily, B7 family, 40 kD
Distribution: B cells and dendritic cells
Function: Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
Ligand/Receptor: ICOS
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Wang S, et al. 2002. J. Biol. Chem. 96:2808. Wong SC, et al. 2003. Blood 102:1831.
Gene ID: 23308
UniProt: View information about CD275 on UniProt.org
Clone: 2D3
Regulatory Status: RUO
Other Names: CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1
Isotype: Mouse IgG2b, κ