Biolegend, 311405, PE anti-human HLA-A,B,C Antibody, 25tests

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8 + T cells. They play an important role in cell-mediated immune responses and tumor surveillance.
25tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Cat, Cow, Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Darrow TL, et al. 1989. J. Immunol. 142:3329. Stern P, et al. 1987. J. Immunol. 138:1088. Tran TM, et al. 2001. Immunogenetics 53:440. Barbatis C, et al. 1981. Gut 22:985. Ayyoub M, et al. 2004. Cancer Immunity 4:7. DeFelice M, et al. 1990. Cell. Immunol. 126:420. Fayen J, et al. 1998. Int. Immunol. 10:1347. Turco MC, et al. 1988. J. Immunol. 141:2275. Geppert TD, et al. 1989. J. Immunol. 142:3763. Wooden SL, et al. 2005. J. Immunol. 175:1383. Nagano M, et al. 2007. Blood 110:151. McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope) Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed Shields MJ. Ribaudo RK. 1998. Tissue Antigens. 51(5):567-70. (epitope)
Product Citations: Hiatt JB, et al. 2022. Clin Cancer Res. 28:4551. PubMed Zhang X, et al. 2022. Cell Rep. 40:111363. PubMed Chimienti R, et al. 2022. Cell Rep. 40:111423. PubMed Fox DB, et al. 2023. NPJ Precis Oncol. 7:25. PubMed Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed Vidard L, et al. 2023. Immun Inflamm Dis. 11:e749. PubMed Shen JZ, et al. 2022. Mol Cell. 82:1123. PubMed Feola S, et al. 2021. ACS Nano. 15:15992. PubMed Meissner T, et al. 2012. Biochem Biophys Res Commun. 418:786. PubMed Oshchepkova A, et al. 2019. Micromachines (Basel). 0.9375. PubMed Mayer RL, et al. 2022. Nat Commun. 13:6075. PubMed Mitchell K, et al. 2018. J Exp Med. 215:1709. PubMed Izumi K, et al. 2021. Immunohorizons. 5:687. PubMed Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed Blank A, et al. 2021. J Pathol. 253:160. PubMed Poznanski SM, et al. 2018. Cancer Immunol Res. 1.065277778. PubMed Zhu XG, et al. 2020. Cell Metabolism. 33(1):211-221.e6. PubMed Skora A, et al. 2015. Proc Natl Acad Sci U S A. 112: 9967-9972. PubMed Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed Zhang J, et al. 2022. Int J Mol Sci. 23:. PubMed Herbring V, et al. 2016. Sci Rep. 6:36907. PubMed Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed Blees A, et al. 2015. Sci Rep. 5:17341. PubMed Nazari-Shafti TZ, et al. 2020. Biomolecules. 10:00. PubMed Hu W, et al. 2012. Asian J Androl. 14:695. PubMed Minervina AA, et al. 2022. Nat Immunol. 23:781. PubMed Saluzzo S, et al. 2022. STAR Protoc. 3:101470. PubMed Singh MV, et al. 2020. J Leukoc Biol. 109:807. PubMed Salzberger W, et al. 2015. PLoS One. 10: 0145324. PubMed Arakawa A, et al. 2021. J Immunol. 207:2235. PubMed Vantourout P, et al. 2014. Sci Transl Med. 6:231. PubMed Miller MS, et al. 2019. J Biol Chem. 294:19322. PubMed Palmer CD, et al. 2022. Nat Med. 28:1619. PubMed Brohl AS, et al. 2021. Cell Rep. 37:110047. PubMed Chida K, et al. 2021. Biol Pharm Bull. 44:1551. PubMed Martufi M, et al. 2019. CRISPR J. 2:31. PubMed Iraqi M, et al. 2020. Front Cell Infect Microbiol. 10:48. PubMed Grossmann N, et al. 2014. Nat Commun. 5:5419. PubMed Merlo A, et al. 2010. J Immunol. 184:5895. PubMed Javitt A, et al. 2019. Front Immunol. 10:141. PubMed Lee PC, et al. 2022. J Clin Invest. 132:. PubMed Strijker JGM, et al. 2021. J Pers Med. 11:. PubMed Chen R, et al. 2021. Front Oncol. 11:743050. PubMed Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
RRID: AB_314874 (BioLegend Cat. No. 311405) AB_314875 (BioLegend Cat. No. 311406)
Structure: Ig superfamily
Distribution: All nucleated cells
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
Gene ID: 3105
UniProt: View information about HLA-A on UniProt.org
Clone: W6/32
Regulatory Status: RUO
Other Names: Major Histocompatibility Class I, MHC class I
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.