Biolegend, 311413, Alexa Fluor® 488 anti-human HLA-A,B,C Antibody, 100tests

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8 + T cells. They play an important role in cell-mediated immune responses and tumor surveillance.
100tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Cat, Cow, Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Darrow TL, et al. 1989. J. Immunol. 142:3329. Stern P, et al. 1987. J. Immunol. 138:1088. Tran TM, et al. 2001. Immunogenetics 53:440. Barbatis C, et al. 1981. Gut 22:985. Ayyoub M, et al. 2004. Cancer Immunity 4:7. DeFelice M, et al. 1990. Cell. Immunol. 126:420. Fayen J, et al. 1998. Int. Immunol. 10:1347. Turco MC, et al. 1988. J. Immunol. 141:2275. Geppert TD, et al. 1989. J. Immunol. 142:3763. Wooden SL, et al. 2005. J. Immunol. 175:1383. Nagano M, et al. 2007. Blood 110:151. McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope) Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed Shields MJ. Ribaudo RK. 1998. Tissue Antigens. 51(5):567-70. (epitope)
Product Citations: Stopfer LE, et al. 2020. Nat Commun. 2760:11. PubMed Sparbier CE, et al. 2023. Nat Cell Biol. 25:258. PubMed Sheridan MA, et al. 2020. Nat Protoc. 15:3441. PubMed Karvas RM, et al. 2022. Cell Stem Cell. 29:810. PubMed Bretscher D 2012. Mol Biol Cell. 1.445138889. PubMed Sheridan MA, et al. 2021. Development. 148:. PubMed Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed Turco MY, et al. 2020. Nature Protocols. 19(5):568-577. PubMed Nobutani K, et al. 2015. PLoS One. 10:130032. PubMed Johnson D, et al. 2016. Nat Commun. 7:10582. PubMed Yeong J, et al. 2019. J Immunother Cancer. 7:34. PubMed Burr ML, et al. 2019. Cancer Cell. 36:385. PubMed Goodridge J, et al. 2013. J Immunol. 191:1567. PubMed Eisemann T, et al. 2019. Neuro Oncol. 1.101388889. PubMed Simmons SK, et al. 2022. Nat Biotechnol. :. PubMed Lee J, et al. 2014. Proc Natl Acad Sci U S A. 111:364. PubMed Larsen BM, et al. 2021. Cell Reports. 36(4):109429. PubMed Lowes L, et al. 2014. J Vis Exp. 84: 51248. PubMed Luo N, et al. 2018. Nat Commun. 0.547222222. PubMed
RRID: AB_493134 (BioLegend Cat. No. 311415) AB_493133 (BioLegend Cat. No. 311413)
Structure: Ig superfamily
Distribution: All nucleated cells
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
Gene ID: 3105
UniProt: View information about HLA-A on UniProt.org
Clone: W6/32
Regulatory Status: RUO
Other Names: Major Histocompatibility Class I, MHC class I
Isotype: Mouse IgG2a, κ