Biolegend, 311909, PE/Dazzle™ 594 anti-human CD267 (TACI) Antibody, 25tests

TACI, Transmembrane Activator CAML (calcium modulator and cyclophilin ligand) Interactor, is a 32 kD type III transmembrane protein. It belongs to TNF receptor superfamily, known as TNFRSF member 13B (TNFRSF13B) or CD267. TACI is expressed on B cells, and myeloma cells. TACI contains 2 cysteine-rich domains (CRDs). Recent studies, however, have shown that another shorter form (TACI_d2) of TACI exists wherein the N-terminal CRD is removed by alternative splicing. TACI_d2 contains full affinity for its ligands. Several proteins (BAFF/BLys, APRIL, Syndecan-2) have been identified as TACI ligands. The interaction of TACI with its ligands induces activation of the transcription factors NFAT, AP1, and NF-κ B and plays a crucial role in humoral immunity by negative regulation of B cell proliferation and survival.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: TACI-transfected RBL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Ng LG, et al.2004. J. Immunol. 173:807. (FC) Lougaris V, et al. 2012. Hum Immunol. 73:836. PubMed.
Product Citations: Dieudonné Y, et al. 2024. Nat Commun. 9921:15. PubMed
RRID: AB_2565250 (BioLegend Cat. No. 311909) AB_2565251 (BioLegend Cat. No. 311910)
Structure: Type III transmembrane protein, TNF receptor superfamily, 32 kD
Distribution: B cells, meyloma cells
Function: Negative regulation of B cell function
Ligand/Receptor: BAFF/BLys, April, Syndecan-2
Cell Type: B cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Gross JA, et al. 2000. Nature 404:995. 2. Wu Y, et al. 2000. J. Biol Chem. 275:35478. 3. Yan M, et al. 2001. Nat. Immunol. 2:638. 4. Hymowitz A, et al. 2005. J. Biol. Chem. 280:7218. 5. Bischof D, et al. 2006. BLOOD 107:3235.
Gene ID: 23495
UniProt: View information about CD267 on UniProt.org
Clone: 1A1
Regulatory Status: RUO
Other Names: TNFRSF13B, CD267, Transmembrane Activator and CAML Interactor (TACI)
Isotype: Rat IgG2a, κ