Biolegend, 314511, PerCP/Cyanine5.5 anti-human IgM Antibody, 25tests

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis. Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Application References(PubMed link indicates BioLegend citation): Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations: Arunachalam PS, et al. 2023. J Clin Invest. 133:. PubMed Dou Y, et al. 2023. Antib Ther. 6:76. PubMed Nunes-Santos CJ, et al. 2023. Nat Commun. 14:3708. PubMed Carre C, et al. 2021. iScience. 24:102970. PubMed Goel RR, et al. 2022. Cell. 185:1875. PubMed Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed Català-Moll F, et al. 2021. Nucleic Acids Res. 49:5057. PubMed Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed van der Burg M, et al. 2019. Front Immunol. 10:246. PubMed Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed Kohn L, et al. 2014. J Immunol. 192:5050. PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Aggarwal C, et al. 2021. J Virol. 95:e0061021. PubMed Flint S, et al. 2016. Haematologica. 101: 698 - 706. PubMed van der Velden V, et al. 2017. J Immunol Methods. 10.1016/j.jim.2017.03.011. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Rother M, et al. 2016. J Immunol. 197: 441 - 448. PubMed Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed van Dongen JJM, et al. 2019. Front Immunol. 10:1271. PubMed
RRID: AB_961367 (BioLegend Cat. No. 314511) AB_2076098 (BioLegend Cat. No. 314512)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3507
UniProt: View information about IgM on UniProt.org
Clone: MHM-88
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.